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It occurs naturally in human cells, where it is one of the body's principal intracellular antioxidants and a substrate in several detoxification pathways

Among the rather long list of their predecessors, the authors mentioned Mortimer Louis Anson (19011968) and Alfred Ezra Mirsky (19001974), who, in 1934, reported that denatured hemoglobin can be digested by trypsin, which does not attack the native protein ( However, the actual father of limited proteolysis as a research tool was Kaj Ulrik Linderstrm-Lang, who, together with Martin Ottesen, coined the term in 1949, to differentiate the restricted specificity of certain enzymes under certain conditions from the random proteolysis accompanying total protein degradation (Linderstrm-Lang and Ottesen, 1949)

Zimmet P, Alberti KG, Shaw J

1 2 "Noonan Syndrome: Genetics and Responsiveness to Growth Hormone Therapy"